Synaptic wiring of hippocampal circuits

  • organotypic hippocampal slice cultures
  • single-cell electroporation
  • AAV injection
  • 2-photon (calcium) imaging
  • patch-clamp electrophysiology
  • optogenetics

Schaffer collateral synapses and hippocampal circuits in vivo

  • cranial window
  • AAV injection
  • chronic 2-photon imaging in vivo
  • CA1 calcium imaging during behavior
  • optogenetics

Development of optogenetic tools

  • molecular engineering
  • molecular cloning
  • organotypic hippocampal slice cultures
  • single-cell electroporation
  • electrophysiology
  • 2-photon imaging